The Tridelta PhaseTM range SAA kit is a solid phase sandwich Enzyme Linked Immuno Sorbent Assay (ELISA). A monoclonal antibody specific for SAA has been coated onto the wells of the microtitre strips provided. Samples, including standards of known SAA content, are added into microwells along with biotinylated anti-SAA monoclonal antibody. Any SAA present in the well is both captured on the plate by the immobilized antibody and labelled with the conjugate antibody in a one step procedure. After washing to remove all of the unbound material, Streptavidin-Horse Radish Peroxidase conjugate is added to the well and incubated. Following the second incubation, TMB substrate solution is added. The intensity of the colour produced is proportional to the concentration of SAA present in the original specimen.
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